Gram Staining Lab Report

Gram Staining Lab Report-46
Keep petri dishes with cultured mediums closed—preferably taped shut—unless sampling or disinfecting.Even then, remove the petri dish only enough to insert your implement or cover medium with bleach or 70% isopropyl alcohol.

Keep petri dishes with cultured mediums closed—preferably taped shut—unless sampling or disinfecting.

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Working with chemicals and bacteria can be hazardous.

Often the chemicals used to prepare slides may be toxic, corrosive or have other related hazards.

Washing them before will minimize contamination of the bacteria cultures you are growing.

Washing them afterwards will minimize your exposure to harmful bacteria that may be growing in your cultures.

After you have tried that, stain each of your live bacteria cultures using the following procedure: Once you have determined which of your live cultures is gram-negative and which is gram-positive, label them clearly and move on to the next part of the experiment.

One way to test bacteria susceptibility to antibiotics is to use the Kirby-Bauer or ‘disc diffusion’ method. What do your results tell you about the process of prescribing antibiotics?

Never eat or drink during bacteria studies, nor inhale or ingest growing cultures.

Work in a draft-free room and reduce airflow as much as possible.

When finished experimenting, seal dishes in a plastic bag and dispose.

Cover accidental breaks or spills with bleach or alcohol for 10 minutes, then carefully sweep up, seal in a plastic bag, and discard.


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